Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody

LRP2 antibody

Boster Bio Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody catalog # M02463. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02463
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody

View all LRP2 Antibodies

SKU/Catalog Number

M02463

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody catalog # M02463. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02463)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EE-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Lrp2 / Megalin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02463 is reactive to LRP2 in Human, Mouse, Rat

Applications

M02463 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

521.958kDa

Background of LRP2

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For LRP2 (Source: Uniprot.org, NCBI)

Gene Name

LRP2

Full Name

Low-density lipoprotein receptor-related protein 2

Weight

521.958kDa

Superfamily

LDLR family

Alternative Names

calcium sensor protein; DBS; DBSlow-density lipoprotein receptor-related protein 2; EC 1.1.2.3; EC 3.4.21.9; Glycoprotein 330; gp330; Heymann nephritis antigen homolog; low density lipoprotein receptor-related protein 2; LRP2; LRP-2; Megalin LRP2 DBS, GP330, LRP-2 LDL receptor related protein 2 low-density lipoprotein receptor-related protein 2|Heymann nephritis antigen homolog|calcium sensor protein|glycoprotein 330|megalin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LRP2, check out the LRP2 Infographic

LRP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody

Question

What is the recommended storage of M02463?

Verified customer

Asked: 2022-06-07

Answer

The Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (M02463) can be stored at -20° C for up to 2 years.

Boster Scientific Support

Answered: 2022-06-07

Question

I see that the anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-10

Question

We are currently using anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

The anti-Lrp2 / Megalin Rabbit Monoclonal antibody (M02463) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-02

Question

Does anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 work on zebrafish WB with cortex of kidney?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Our lab technicians have not tested anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cortex of kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-25

Question

What kind of running buffer was used for M02463?

Verified customer

Asked: 2019-08-23

Answer

Our lab used AR0139 as running buffer for the Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (M02463). https://www.bosterbio.com/tris-glycine-sds-running-buffer-pack-ar0139-boster.html

Boster Scientific Support

Answered: 2019-08-23

Question

Has lysate0032 been tested to express LRP2 by using M02463 antibody?

Verified customer

Asked: 2019-07-18

Answer

Yes, our lab technicians confirmed that the Human 293T Whole Cell Lysate LYSATE0032 expressed LRP2 by using Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody M02463 during our test.

Boster Scientific Support

Answered: 2019-07-18

Question

We ordered #M02463 Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody, last week. Please let us ask you about your Rabbit monoclonal antibodies. 1. Are your rabbit monoclonal antibodies recombinant antibodies? What kind of host cells was used to express each recombinant antibody? 2. How was the transgene, the variable region gene, introduced into the host cells? Plasmid transfection, viral infection or any other way?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

Please see the following answers for your questions. 1. Are your rabbit monoclonal antibodies recombinant antibodies? What kind of host cells was used to express each recombinant antibody? M02463 is a recombinant protein. Host cell is 293 eucaryote cell. 2. How was the transgene, the variable region gene, introduced into the host cells? Plasmid transfection, viral infection or any other way? This information is confidential. Sorry we can not disclose it.

Boster Scientific Support

Answered: 2019-07-05

Order DetailsPrice
M02463

100μL

$370
M02463-30ul

30μL sample

$99
M02463-carrier-free

Carrier Free

$370

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M02463
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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